Abstract
Nine kinds of synthetic antibacterials being used as feed additives were separated into four fractions by chromatography on an ion exchange column and a florisil column. Clopidol, caprylohydroxamic acid, sulfaquinoxaline and nicarbazine were adsorbed on Amberlite GC-400 (OH) from the methanol extract of chiken muscle or egg and were eluted with 25ml of a mixture of 10% sodium chloride, hydrochloric acid and methanol (10:0.5:90) as fraction I. Washings and effluent containing unadsorbed materials were passed through a florisil column. Dinitolmide and ethopabate were eluted with 100ml of ethylether as fraction II, robenidine and decoquinate were eluted with 20ml of 1% calcium chloride in methanol as fraction III, and then amprolium was eluted with 25ml of 18% potassium chloride in methanol as fraction IV.Each fraction was concentrated and analyzed by gas chromatography except for fraction IV, which was analyzed fluorometrically for amprolium.
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