Simultaneous analysis of photoinduced electron transfer in wild type and mutated AppAs

Abstract

Photoinduced electron transfer (PET) from Tyr21 to isoalloxazine (Iso) in the excited state (Iso*) is considered to be an initial step of the photosensing function of the blue-light sensing using flavin adenine dinucleotide (BLUF) component of the anti-repressor of the photosynthetic regulation (AppA). The PET mechanism was investigated via fluorescence dynamics of AppA and Kakitani and Mataga (KM) theories as well as by molecular dynamic (MD) simulation. The local structures of both the Y21F and W104F mutant AppAs around the Iso binding sites were quite different from those of the wild type (WT) AppA. The distances between Iso and Trp104 in Y21F, and between Iso and Tyr21 in W104F were shorter by 0.06 nm and 0.02 nm, respectively, compared to the WT. The frequency factor, ν 0, in Tyr21 was 1.15-fold greater than that in Trp104. The critical distance between adiabatic and non-adiabatic PET processes, R 0, was found to be very long in the AppA Tyr21. The large values of ν 0 and R 0 for Tyr21 of AppA compared to those in a non photosensing flavoprotein, FMN binding protein (FBP), were elucidated by hydrogen bond (H bond) chain between Tyr21 and Iso through Gln63. Interaction energies among Iso*, Trp104, Tyr21 and Gln63 in WT were calculated using the semi-empirical PM3 method. The amount of the transferred charge from Trp104 to Iso* in the WT exhibited a maximum at an interaction energy of around −20 kcal/mol, but decreased as the interaction energy (absolute value) increased.