Abstract
A method was developed to separate and simultaneously quantitate phosphatidylglycerol (PG), phosphatidylinositol (PI), phosphatidylethanolamine (PE), phosphatidylcholine (PC), sphingomyelin (SM) and lysophosphatidylcholine (LPC) in rabbit bronchoalveolar lavage fluid (BALF) by liquid chromatography/mass spectrometry (LC/MS). This method consisted of a simple liquid-liquid extraction procedure, separation of phospholipid classes on silica gel column by gradient mode, and detection of mass spectrometry with electrospray ionization (ESI). The precision, accuracy and recovery ranged from 1.6 to 7.6%, −0.8 to +14.7% and 69.3 to 90.0%, respectively. This method was applied to compare the content and the composition of phospholipid classes in BALF collected from inflammation-model and control rabbit. The ratio of LPC concentration to PC significantly increased in inflammation-model BALF compared with control BALF.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
