Abstract
Morinda officinalis is an important herbal medicine and functional food, and its main constituents include anthraquinone and iridoid glycosides. Quantification of the main compounds is a necessary step to understand the quality and therapeutic properties of M. officinalis, but this has not yet been performed based on liquid chromatography/tandem mass spectrometry (LC-MS/MS). Analytes were extracted from M. officinalis by reflux method. Ultrahigh-performance liquid chromatography coupled with a triple quadrupole mass spectrometry (UPLC-QqQ-MS) using multiple reaction monitoring (MRM) mode was applied for quantification. Fragmentation pathways of deacetyl asperulosidic acid and rubiadin were investigated based on UPLC with quadrupole time-of-flight tandem mass spectrometry (Q/TOF-MS) in the MSE centroid mode. The method showed a good linearity over a wide concentration range (R2 ≥ 0.9930). The limits of quantification of six compounds ranged from 2.6 to 27.57 ng/mL. The intra- and inter-day precisions of the investigated components exhibited an RSD within 4.5% with mean recovery rates of 95.32–99.86%. Contents of selected compounds in M. officinalis varied significantly depending on region. The fragmentation pathway of deacetyl asperulosidic and rubiadin was proposed. A selective and sensitive method was developed for determining six target compounds in M. officinalis by UPLC-MS/MS. Furthermore, the proposed method will be helpful for quality control and identification main compounds of M. officinalis.
Highlights
IntroductionIts roots—an important traditional Chinese medicine (TCM) and functional food—have been widely used for the treatment of sexual impotence, spermatorrhea, irregular menstruation, and female infertility for more than 2000 years [2,3]
These results indicated that there were significant differences in the contents of monotropein, deacetyl asperulosidic acid, asperulosidic acid, asperuloside, rubiadin-1-methyl ether, and rubiadin among the 17 samples
It was successfully applied to simultaneously quantify the six bioactive components in 17 batches of M. officinalis samples collected from different regions of China
Summary
Its roots—an important traditional Chinese medicine (TCM) and functional food—have been widely used for the treatment of sexual impotence, spermatorrhea, irregular menstruation, and female infertility for more than 2000 years [2,3]. Pharmacological studies have revealed that M. officinalis exhibits antiosteoporosis, antifatigue, antioxidative, antidepressant, and hypoglycemic activities [4,5,6,7]. Anthraquinones, polysaccharides, and oligosaccharides are the main bioactive constituents of M. officinalis [8,9]. In Chinese Pharmacopoeia (2015 edition), nystose assay using high-performance liquid chromatography (HPLC) and thin layer chromatography (TLC) identification was performed for quality control of M. officinalis [3]. Herbal medicines exert their curative effects through multiple components on multiple targets [10].
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