Simultaneous analysis of eprosartan and hydrochlorothiazide in tablets by high-performance thin-layer chromatography with ultraviolet absorption densitometry

Abstract

Summary A new, simple, accurate, and precise high-performance thin-layer chromatographic (HPTLC) method has been established for simultaneous analysis of eprosartan and hydrochlorothiazide in tablet formulations. Standard and sample solutions of eprosartan and hydrochlorothiazide were applied to precoated silica gel G 60 F254 HPTLC plates and the plates were developed with benzene-methanol-formic acid 7:3:0.1 (υ/υ) as mobile phase. Detection and evaluation of chromatograms was performed densitometrically at 272 nm. The retention factors of eprosartan and hydrochlorothiazide were 0.76 and 0.57, respectively. The linear range was 4.8–43.2 μg per spot for eprosartan and 0.15–1.35 μg per spot for hydrochlorothiazide; the correlation coefficients, r, were 0.998 and 0.999, respectively. The method was validated and successfully used for analysis of the drugs in tablets.