Simultaneous analysis of blasticidin S and kasugamycin with micellar liquid chromatography

Abstract

A simple and fast micellar liquid chromatographic (MLC) method for simultaneous analysis of the antibiotic fungicides of blasticidin S and kasugamycin was developed. Chromatographic separation was achieved on a Phenomenex® Aqua 5μ C 18 125 A column. The mobile phase consisted of an aqueous mixture of 69.3 mM sodium dodecyl sulfate (SDS) with deionized water (50 : 1, v/w). The flow rate was set at 1.0 mL/min, and the absorbance was monitored at 210 nm with a photodiode array detector. The total run time was less than 10 min, and the retention times (t r) were 2.47 min for blasticidin S ion, and 7.15 min for kasugamycin ion. Good linear relationships were obtained with correlation of coefficients (r) of 0.997 for kasugamycin, and 0.999 for blasticidin S. The limits of detection (LODs) of blasticidin S and kasugamycin were 0.5 μg/mL and 0.75 μg/mL, respectively, and the limits of quantitation (LOQs) for blasticidin S and kasugamycin were 1.5 μg/mL and 2.2 μg/mL, respectively. The recoveries of blasticidin S and kasugamycin in blank irrigation water ranged from 94.9-97.3% and 87.8-99.7%, respectively. By using MLC method blasticidin S and kasugamycin could be analyzed simultaneously.