Abstract
A simple and simultaneous analysis method for four (anionic, amphoteric, nonionic, and cationic) classes of surfactants in shampoo and hair conditioner was newly developed. Analysis of the surfactants was performed using a reversed-phase HPLC (RPLC) combined with evaporative light scattering detection (ELSD) without any pre-treatment. An optimum analysis condition for the resolution of both four main surfactant mixtures used in shampoo and five main surfactants used in hair conditioner could be established under a gradient mobile phase condition with acetonitrile, tetrahydrofuran and water. The detection limits were 2.5–30 μg mL −1 except for SLES (150 μg mL −1), and the calibration curves, i.e. the log–log plots, were linear in the working range of 2.5–5250 μg mL −1 with R 2 values of above 0.998. The observed precision was less than 5% R.S.D. The elution peaks were identified by a liquid chromatography–mass spectrometer (LC–MS) equipped with an electrospray interface operating in mixed-mode.
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