Simultaneous anaerobic decolorization/degradation of Reactive Black-5 azo dye and chromium(VI) removal by Bacillus cereus strain MS038EH followed by UV-C/H2O2 post-treatment for detoxification of biotransformed products.

Abstract

The existence of synthetic dyes and heavy metals in textile wastewater is a serious problem. These compounds should be removed before discharge into the environment by an appropriate method. The present study was conducted for the characterization of efficient multi-functional strain Bacillus cereus MS038EH for the simultaneous removal of Reactive Black-5 and Chromium(VI). Maximum decolorization efficiency of 94.74% was achieved at pH 7, 35°C, and 4% inoculum size for 900mg/L of Reactive Black-5. Also, 94.10% efficiency was observed in the presence of 8g/L of yeast extract as an optimum nitrogen source, while carbon sources had no significant effect on decolorization. It should be pointed out that the decolorization efficiency was decreased from 94 to 64% by increasing NaCl concentrations from 0 to 50g/L, respectively. Bacillus cereus strain MS038EH could decolorize 94.31% of Reactive Black-5 (900mg/L) and remove 87.31% of chromium(VI) (30mg/L) within 36h. Results of Gas chromatography-mass spectrometry and Fourier transform-infrared spectroscopy proved that Reactive Black-5 was cleaved into the lower molecular weight products without any azo bonds. However, the phyto-toxicity analysis showed that Reactive Black-5 was not toxic for Triticum aestivum and Maize, while biologically treated Reactive Black-5 was toxic for seeds. Therefore, ultraviolet-C/H2O2 was applied for the detoxification of biotransformed products. When ultraviolet-C/H2O2 was applied as post-treatment, the seeds were germinated completely. It is demonstrated that the application of ultraviolet-C/H2O2 after anaerobic treatment is effective for toxicity reduction of textile wastewater.