Abstract
Department of Physics and Astronomy, Iowa State University, Ames, Iowa 50011. Abstract Single Molecule Fluorescence Resonance Energy Transfer (FRET) and single molecule force measurements with the Atomic Force Microscope (AFM) are two powerful techniques that have facilitated much progress in the biological sciences. However each of these techniques suffers from limitations that can be overcome by the use of a combined single molecule AFM-FRET approach. Here, we describe an instrument that successfully combines single molecule AFM with FRET to apply forces on individual biological molecules and simultaneously monitor their conformational dynamics. To validate this technique, we measured the force induced shearing of dye-labeled, double stranded DNA. Single DNA molecules were sheared and mechanical transitions corresponding to DNA rupture were correlated with changes in FRET.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
