Simulations of edge behavior in a mixed-lipid bilayer: Fluctuation analysis

Abstract

Coarse-grained molecular dynamics simulations of lipid bilayer ribbons consisting of a mixture of lipids of different tail lengths have been performed to gain insight into bicelle mixtures. The line tension of the bilayer edge decreases as the mole fraction of short-chain lipids in the system is increased, dropping below zero between 30% and 35%. The mole fraction of short-chain lipids in the ribbon interior is lower than the total mole fraction, as the short-chain lipids segregate towards the edge, but continues to rise even after the line tension vanishes, in contrast to predictions of a two-component two-phase model. The fluctuations of the bilayer edge in both high and low line tension regimes have been analyzed to extract information about the factors that influence the length and shape of the edge. At high line tension the wavelength-dependent in-plane fluctuations of the edge are predicted quantitatively using a simple analytical model using only the line tension as input. Where line tension is vanishing, the fluctuations can be modeled as arising from a combination of harmonic fluctuations around a minimum energy contour length and an in-plane bending elasticity. The estimated value of the in-plane bending modulus is of order 10(-29) J m, placing the intrinsic persistence length for the edge near the bilayer thickness of 4 nm.