Abstract
To measure the activity of tissue at the microscopic level, laminar optical tomography (LOT), which is a microscopic form of diffuse optical tomography, has been developed. However, obtaining sufficient recording speed to determine rapidly changing dynamic activity remains major challenges. For a high frame rate of the reconstructed data, we here propose a new LOT method using compressed sensing theory, called compressive laminar optical tomography (CLOT), in which novel digital micromirror device-based illumination and data reduction in a single reconstruction are applied. In the simulation experiments, the reconstructed volumetric images of the action potentials that were acquired from 5 measured images with random pattern featured a wave border at least to a depth of 2.5 mm. Consequently, it was shown that CLOT has potential for over 200 fps required for the cardiac electrophysiological phenomena.
Highlights
Fluorescence tomography is a useful tool for investigating volumetric distributions of tumors and other features in three-dimensional biological tissue
To improve the frame rate of Laminar optical tomography (LOT) in comparison with the conventional tomography technique, we propose compressive laminar optical tomography (CLOT), which is a form of LOT using patterned illumination based on CS
In comparison with the fluorophore that exists locally, the distributions of the action potentials mainly have the direct current (DC) component of the spatial frequency, and more number of coefficients were required to represent the distribution;.these distributions cannot be represented as the sufficient sparse matrices by using DCT and discrete wavelet transform (DWT), and the reconstruction results obtained from DCT, Haar 1.3, and Haar 3.3 were similar in the prepared distributions
Summary
Fluorescence tomography is a useful tool for investigating volumetric distributions of tumors and other features in three-dimensional biological tissue. The conventional LOT method uses raster scanning, which involves measurement at each point in the tissue and has a low recording speed, mainly due to the mechanical speed limitation of the GMs and the low intensity of the fluorescence to be detected [1, 3,4,5,6] In this approach, there is a trade-off between the recording speed and the spatial resolution of the system. In order to achieve fast three-dimensional mesoscopic imaging, a time-resolved angled fluorescence laminar optical tomography (aFLOT) system in combination with voltage-sensitive dye imaging has been used [9, 10] This system works based on line illumination and has a charge coupled device (CCD) camera, and this can measure the area of the line illumination at 200 fps. Measuring the aperiodic fast activity of tissue still remains difficult
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
