Abstract

ABSTRACT We apply a previously developed 3D Finite-Difference Time-Domain (FDTD) simulation method to model the optical phase contrast microscopic (OPCM) visualization of Gold nanoparticles (NPs) attached to the nucleus of a singe biological cell. We consider a realistic size 3D cell model at optical immersion conditions, i.e. when the refractive index values of the cytoplasm and of the extra-cellular medium are eq ual. The visual effect of the Gold NPs is studied at both resonant and non-resonant conditions. The results show that, at resonance, there are specific visual patterns that could be used for the identification of the presence of NPs at the nu cleus’ surface. The model demons trates the capability to model the specific conditions of OPCM image enhancement by optically controlling the resonant properties of the NPs. Keywords: Biological cell, finite-difference time-domain method, optical phase contrast microscope, optical clearing effect, Gold NPs 1. INTRODUCTION The application of optical software simulation and modeling tools in biomedical optics is a growing area. Biomedical optics researchers need optical simulation tools to acquire a deeper understanding of the light-cell-tissue interaction processes.

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