Abstract
This paper describes a novel design and a simple, low cost fabrication method of concentration gradient generator which can generate the approximate linear and quadratic concentration gradient curves output. The concentration gradient generators were fabricated in polymethyl-methacrylate (PMMA). The gradient curves can be controlled by the flow velocities. The generator is designed and simulated using CAXA and COMSOL multiphysics, and the concentration gradient generated in the simulation and experiment was analyzed through Origin 8.0 software. The generator was fabricated with the CO2 laser processing machine and thermocompressor. We have carried out a deep research on the processing parameters of laser machine and got an ideal microchannel effect and the microchannels are processed using the CO2 laser processing machine on PMMA substrate. In addition, we also proposed a method of multiple bond to bond chip. Finally, the feasibility of simulation was clearly verified by a mixing experiment with blue ink and yellow ink. This simple microfluidic device can help the study of high-throughput concentration gradient applications such as cell biology of chemotaxis or hypotaxis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
