Simulated gastrointestinal digestion of yak bone collagen hydrolysates and insights into its effects on gut microbiota composition in mice

Abstract

In this study, the in vitro simulated gastrointestinal digestion and a thirty-days in vivo diet intervention of yak bone collagen hydrolysates (YBCH) were conducted to evaluate the bioavailability of YBCH after digestion and its effects on the composition of gut microbiota. Results indicated that there was no intense alteration in the molecular weight distribution of peptides during the simulated digestion. At the phylum level, the ratio of Firmicutes to Bacteroidetes was significantly decreased 30.8% and 36.5% in the low-dose (LD) and medium-dose (MD) groups, respectively. The total concentration of short-chain fatty acids in the intervention groups was increased by 50%. At the end of the trial, compared with the normal control group, the bodyweight of mice in the LD and MD groups was separately reduced by 3.07% and 4.38%. The obtained outcomings would promote the application of YBCH in the field of functional food.