Abstract
Design modification of a particle inflow-type gun for particle bombardment significantly simplifies construction and reduces fabrication time. The gun consists of a high-speed electric solenoid valve mounted on and through a vacuum jar. DNA-coated tungsten particles are placed on the support grid of a filter housing and accellerated by a burst of pressurized helium, which is controlled by a timer. Specimens are held between plastic screens and their distance from the particle support grid is adjusted with a miniature laboratory apparatus positioner. Transient expression of GUS in cantaloupe cotyledons and grape somatic embryos was equivalent to that obtained with a conventional particle inflow gun. The device was constructed with locally-available hardware in 40 minutes using a hand drill, some thread taps and a thread die.
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