Abstract
This paper described an organic solvent-free, simple, and small scale method of sample preparation followed by high-performance liquid chromatography (HPLC) coupled photo-diode array (PDA) detector for quantifying ractopamine (RP) in beef. The HPLC-PDA was performed on an Inertsil ® HILIC, hydrophilic interaction chromatographic mode, column with an isocratic aqueous mobile phase. An analyte was extracted from the sample using a handheld ultrasonic homogenizer with trichloroacetic acid solution and purified by MonoSpin ® SCX, a centrifugal monolithic silica spin mini-columns, and quantified within 25 min. The proposed method obtained average recoveries for the analyte in the range of 95.2 - 103.1% with relative standard deviations ≤ 2.8%. The quantitation limit was 10 ng g -1 in the flank and liver.
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