Abstract
Abstract A solid-phase radioimmune assay (RIA) has been used to subtype serum specimens containing Australia antigen (HB antigen) as either ay or ad . The radioimmune assay system permitted the correct identification of 24 coded specimens that had previously been subtyped by agar gel immunodiffusion. In a series of 52 sera from soldiers with HB antigen positive hepatitis, only 7 (13 per cent) could be subtyped by AGD, (Agar gel diffusion) whereas 33 (64 per cent) were subtyped by RIA. Twenty-eight 54 per cent) were ad and 5 (10 per cent) were ay . Solid-phase RIA methodology provides a means of subtyping many HB antigen positive specimens that cannot be subtyped by other methods.
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