Simplification and optimization of media ingredients for enhanced production of CMCase by newly isolatedBacillussubtilisNA15

Abstract

Cellulose degrading bacterium was isolated from composting biomass and identified as Bacillus subtilis on the basis of phenotypic fingerprint using GEN III MicroPlateTM and 16S ribosomal RNA (rRNA) gene sequence analysis. 16S rRNA gene sequence similarity analysis showed 99% similarity with Bacillus subtilis. The screening of 11 nutrients was done using Plackett-Burman design to explicate the parameters that significantly influence the carboxy methyl cellulase (CMCase) production. Maximum CMCase production of 0.47 U/mL was obtained using response surface methodology with carboxy methyl cellulose (CMC): 18 g/L, peptone: 5 g/L, yeast extract: 5 g/L and MnCl2: 0.5 g/L. The model predicted the maximum CMCase activity (0.45 U/mL), which was in good agreement with the experimental value of 0.47 U/mL showing sevenfold increase as compared to unoptimized medium. Presence of MnCl2 in the medium significantly enhanced the CMCase production. © 2014 American Institute of Chemical Engineers Environ Prog, 34: 533–541, 2015