Abstract
We constructed a simple, versatile, and practical impedimetric immunosensor for virus detection. The gold nanoparticles (AuNPs) were electrostatically bound to polyaniline (PAni) and subsequently deposited on the surface of the PAni-coated gold electrode. The PAni/AuNPs nanocomposites layer improves the sensor's electroactivity and provides a scaffold to conjugate the antibodies against the targeting virus. The step-by-step fabrication of the immunosensor was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The fabricated sensor was optimized for detecting Norovirus-like particles (NoV-LPs) as a model virus. The sensor displayed a detection limit of 1.80 fg/mL, high specificity, and less than 10% of the coefficient of variation of the detection signal. To prove the sensor's versatility and evaluate its applicability, the anti-Dengue virus (DENV) non-structural protein 1 (NS1) antibody was conjugated into the surface of the electrode. This sensor successfully detected clinical DENV-NS1 samples from patients and discriminated between positive and negative Dengue infection. It indicates the feasibility of the practical immunosensor for virus detection and its potency for large-scale fabrication.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
