Simple surface modification of polypropylene pipette tips for anchoring of organic monolithic-based materials for micro-solid-phase extraction

Abstract

This paper presents a simple method for the surface modification of polypropylene pipette tips by adsorbing a photo-initiator, 2,2-dimethoxy-2-phenylacetophenone (DMPAP), to create reactive sites for the formation of a layer of ethylene dimethacrylate (EDMA) and subsequent monolith polymerization. The types of monomers and the degree of crosslinking dramatically affected the monolith shrinkage and detachment in unmodified tips. Effective surface modification for anchoring monolithic materials to pipette tips was achieved using 15 wt% DMPAP and 10 wt% EDMA in methanol with UV irradiation at 365 nm. The extraction of 5-hydroxyindoleacetic acid, serotonin, and bisphenol A (BPA) using methacrylate and activated charcoal composite monoliths was investigated in terms of breakthrough capacity. The application of monolithic pipette tip micro-solid-phase extraction followed by HPLC-UV was demonstrated for determining BPA leaching from baby-feeding bottles and canned foods. Wide linearity ranging from 0.1 to 100 ng mL−1 (R2 = 0.9998) with good repeatability (% RSD = 3.9 %) and accuracy (% recovery = 93–106 %) was obtained. The limit of detection and limit of quantification were 0.084 and 0.280 ng mL−1, respectively. By varying the sample loading volume from 0.50 to 10.00 mL with eluting volume of 150 μL, a 2-to-52-fold pre-concentration factor was observed.