Abstract
Zika virus (ZIKV), a typical example of a re‐emerging pathogen, recently caused large outbreaks in Pacific islands and the Americas, associated with congenital diseases and neurological complications. Deciphering the natural history, ecology and pathophysiology of this mosquito-borne pathogen requires effective reverse genetics tools. In the current study, using the bacterium-free ‘Infectious Subgenomic Amplicons’ (ISA) method, we generated and made available to the scientific community via the non-profit European Virus Archive collection, two simple and performing reverse genetics systems for ZIKV. One is based on an Asian ZIKV strain belonging to the outbreak lineage (French Polynesia 2013). The second was designed from the sequence of a low-passaged ZIKV African strain (Dakar 1984). Using the ISA procedure, we derived wild-type and a variety of specifically engineered ZIKVs in days (intra- and inter-lineage chimeras). Since they are based on low-passaged ZIKV strains, these engineered viruses provide ideal tools to study the effect of genetic changes observed in different evolutionary time-scales of ZIKV as well as pathophysiology of ZIKV infections.
Highlights
Zika virus (ZIKV), a typical example of a re‐emerging pathogen, recently caused large outbreaks in Pacific islands and the Americas, associated with congenital diseases and neurological complications
Using the Infectious Subgenomic Amplicons’ (ISA) procedure, we derived wild-type and a variety of engineered ZIKVs in days. Since they are based on low-passaged ZIKV strains, these engineered viruses provide ideal tools to study the effect of genetic changes observed in different evolutionary time-scales of ZIKV as well as pathophysiology of ZIKV infections
ZIKV re-emerged in the Pacific Ocean islands in 2013, causing outbreaks in French Polynesia[4], which was followed by a series of ZIKV outbreaks affecting numerous Pacific Ocean islands[5,6]
Summary
Thérèse Atieh[1], Cécile Baronti[1], Xavier de Lamballerie1,2 & Antoine Nougairède[1,2] received: 12 September 2016 accepted: 22 November 2016 Published: 19 December 2016. Zika virus (ZIKV), a typical example of a re‐emerging pathogen, recently caused large outbreaks in Pacific islands and the Americas, associated with congenital diseases and neurological complications. Using the ISA procedure, we derived wild-type and a variety of engineered ZIKVs in days (intra- and inter-lineage chimeras) Since they are based on low-passaged ZIKV strains, these engineered viruses provide ideal tools to study the effect of genetic changes observed in different evolutionary time-scales of ZIKV as well as pathophysiology of ZIKV infections. By generating chimeric ZIKV strains (intra and inter-lineage chimeras), we demonstrate that this procedure conveniently allows the generation of genetically modified ZIKVs in days and will be a major tool for identifying genetic determinants associated with the recent expansion of the virus and its increased virulence
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