Abstract
Featured Article: Nagatsu T and Udenfriend S. Photometric assay of dopamine-β-hydroxylase in human blood. Clin Chem 1972;18:980–3.1 This report described a simple assay for measuring the activity of dopamine-β-hydroxylase (DBH2 ) in human blood. DBH is a copper-containing, ascorbate-requiring glycoprotein enzyme that catalyzes the hydroxylation of dopamine to norepinephrine (NE), the precursor of epinephrine (E). DBH is localized in the synaptic vesicles in the central NE and E neurons and peripheral sympathetic NE neurons and in the chromaffin granules in E and NE cells of the adrenal medulla; DBH is released by exocytosis together with NE or E, and appears in both blood and cerebrospinal fluid (CSF). Since I was a medical student at Nagoya University, Japan, I have been interested in the chemical mechanisms of neuropsychiatric diseases and, for that reason, in the biochemistry of catecholamine neurotransmitters that are assumed to be closely related to various brain diseases. I first worked with Sidney Udenfriend in 1964 at NIH, where we discovered tyrosine hydroxylase, the rate-limiting enzyme in the biosynthesis of catecholamines. Studies …
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