Simple methods of dissection protocols for the rapid isolation of rat dorsal root ganglia under the non-microscopic condition

Abstract

The dorsal root ganglia (DRG) neurons have long been studied to advance the understanding of sensory nerve function and growth. DRG neurons are also involved in determining the fundamental mechanisms of neurodegenerative and painful disorders of the peripheral nervous system. To explore the simple methods of rapid and accurate removal of DRG in anesthetized rats with the naked eye without destroying the spinal column or blood circulation. Male Wistar rats were fixed on the operating table, lumbar DRG was removed, and sufficient DRG neurons were harvested without microscopic equipment. At the same time, the procedure was accomplished without disruption to breathing and circulation. These approaches reduce the time required for DRG collection, thereby improving efficiency, and increasing the chances of generating healthy primary DRG cultures and reproducible experiments using DRG tissue.