Abstract
The reversed phase chromatography of ten amino acids(serine, glutamic acid, glycine, alanine, valine, methionine, leucine, isoleucine, phenylalanine, proline) in Japanese sake was studied by using a copper(II) ion selective electrode as detector. Chromatographic conditions were: column, TSKgel ODS-120A (particle size 5μm, 4.6mm i.d.×25cm); mobile phase, waterethanol (95:5 v/v); flow rate, 0.23cm3min-1. The copper(II) reactant solution contained 10-5 mol dm-3 copper nitrate, 0.05mol dm-3 3-(N-morpholino) propanesulfate buffer(pH 7.0), 0.05 mol dm-3 ammonium acetate and 1%(w/v) potassium nitrate, and was delivered at the flow rate of 0.27cm3min-1. The change of copper(II) ion activity by the post-column reaction between copper(II) reactant solution and amino acid was monitored with a copper(II) ion selective electrode (DKK 7141). Eight peaks were obtained, serine, glycine and alanine were not resolved. Calibration curves of seven amino acids were linear for 0.21.5μg/10mm3. Amino acids in Japanese sake could be simply determined.
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