Abstract
This paper presents a novel approach to the long-term recording of electrocardiogram (ECG) signals in prawns, including a 3-lead electrode, a lightweight external wires assembly, a waterproof electrode-wire junction, and a standardized implanting procedure. The proposed low-cost device is easily constructed, even by untrained undergraduate students, using common laboratory materials. Consistent ECG recordings were obtained over a period of 72 h in experiments on 6 freely moving prawns (Macrobrachium rosenbergii). A subsequent experiment conducted continuously for 38 d revealed that despite gradual attenuation the ECG signals could still be identified (signal-to-noise ratio ≥3). The prawns survived implantation and later underwent natural molting, whereupon the electrode was successfully re-implanted. The proposed electrode is a valuable tool by which to gain insight into the long-term physiological state of crustaceans.
Highlights
Decapod crustaceans are a diverse group of more than 14 000 extant species, many of which are exploited commercially as a source of protein (Deshmukh 2013)
Developing a more complete understanding of decapod crustaceans is crucial to the sustainability of this resource
This paper presents a novel approach to the longterm recording of ECG signals in prawns, including a 3-lead electrode with lightweight external wires and waterproof electrode−wire junctions providing buoyancy
Summary
Decapod crustaceans (e.g. crabs, prawns, lobsters, and crayfish) are a diverse group of more than 14 000 extant species, many of which are exploited commercially as a source of protein (Deshmukh 2013). Based on heart rate, Cooper et al (2011) monitored the physiological conditions in crustaceans of both sexes during copulation. Yazawa & Katsuyama (2001) reported that their approach to ECG recording could be continued for several weeks; no mention was made as to the quality of ECG recordings over time Those articles did not provide a clear description of the position of the electrode leads implanted in the animal’s body (i.e. using vague terms, such as cardiac region). This paper presents a novel approach to the longterm recording of ECG signals in prawns, including a 3-lead electrode with lightweight external wires and waterproof electrode−wire junctions providing buoyancy. Electrode implantation appears to have only a minor physiological impact on the animals, as indicated by continued normal growth and molting following the operation
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