Simple Method for Determination of Short-Chain Organic Acid in Mead

Abstract

Organic acids present in mead influence its fermentation rate during the production process, its stability and microbiological purity as well as organoleptic properties of the final product. The objective of this work was to validate an analytical method for determination of eight main organic acids in mead using the isotahophoresis with conductivity detection. All the validation parameters indicate high reliability of the method. The recovery rates of all analytes were between 96 and 101 %. The precision was equal or less than 2.5 %. The isotachophoretic system showed also high sensibility due to low limits of detection and quantification. For all investigated organic acids, limit of detection (LOD) ranged from 0.7 to 4.7 mg L−1 and limit of quantification (LOQ) values ranged between 2.2 and 14.2 mg L−1.