Abstract
AbstractWe established a simple method for determination of oxalate in forage grasses using high‐performance liquid chromatography (HPLC) with an anion analysis column (Shodex 1C SI‐90 4E) and sodium hydrogen carbonate (NaHCO3) as eluent. Oxalate was determined after extraction with water or 1 mol HCl (soluble form or total, respectively). Insoluble oxalate was estimated as the difference between total oxalates and soluble form. Recovery of oxalate after HPLC was above 97%. Oxalates in forages could be successfully determined by this method. The method was simple, fast and inexpensive.
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