Abstract
A convenient method has been devised to chromatograph simultaneously numerous samples of reassociated deoxyribonucleic acid under thermoregulated conditions. Small amounts of hydroxyapatite (0.2-ml bed) were used, and the effluent volumes for separating single-stranded deoxyribonucleic acid from deoxyribonucleic acid duplexes were 1.5 ml each. The method was comparable in reliability and reproducibility to other well-established techniques, but it combined improvements in ease of operation, in rapidity, and in simplicity, which are desirable in large taxonomic studies.
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