Abstract
Embryo transfer has been used as one of the essential reproductive technologies for production of new strains and maintenance of genetic resources in animals. Mating with vasectomised male rats is a requirement for inducing pseudopregnancy in female rats selected for embryo transfer. Although this procedure has been used routinely, large breeding space and high expenditure are required to maintain a sufficient number of females and vasectomised males. This study was performed to induce pseudopregnancy in females by artificial stimulation using sonic vibration instead of vasectomised males. The females continued to be in the dioestrus stage for at least 14 days after artificial stimulation was performed. Of fresh 2-cell embryos that transferred into the oviducts of females after artificial stimulation, 56% was implanted and 50% was developed to offspring. Approximately 46% of the frozen 2-cell embryos were implanted and 24% developed into offspring. Furthermore, 66% of the fresh pronuclear embryos were implanted and 60% developed into offspring. This study successfully induced pseudopregnancy in rat females by artificial stimulation using a sonic vibration. This method, ‘Easy-ET’, was useful for efficient production and maintenance of rat strains.
Highlights
Embryo transfer has been used as one of the essential reproductive technologies for production of new strains and maintenance of genetic resources in animals
Embryo transfer (ET) is required for the efficient production of new strains from these genome-edited embryos and the regeneration of valuable strains from frozen embryos that are used as genetic resources[18,19,20]
The results showed that pseudopregnancy was induced by artificial stimulation using sonic vibration
Summary
Embryo transfer has been used as one of the essential reproductive technologies for production of new strains and maintenance of genetic resources in animals. This study was performed to induce pseudopregnancy in females by artificial stimulation using sonic vibration instead of vasectomised males. This study successfully induced pseudopregnancy in rat females by artificial stimulation using a sonic vibration This method, ‘Easy-ET’, was useful for efficient production and maintenance of rat strains. Transfer of 2-cell embryos into oviducts is being routinely used as a stable standard technology for the efficient production of genetically engineered strains and removal of pathogens in laboratory animals. We successfully performed simple induction of pseudopregnancy by artificial stimulation using a sonic vibration instead of vasectomised males. The embryos transferred into the oviducts of these pseudopregnant females developed into healthy offspring
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