SIMPLE HPLC METHOD FOR THE QUANTIFICATION OF GINGEROLS (4-, 6-, 8-, AND 10-) AND SHOGAOLS (6-, 8-, AND 10-) IN Zingiber officinale var. rubrum SUPERCRITICAL CARBON DIOXIDE (SC-CO2) EXTRACT

Abstract

Zingiber officinale var. rubrum is known as ‘Halia bara’ in the Southeast Asia region. Many herbal products containing Halia bara are in the market are being used for either cosmetic purposes or well-being. The present study aims to develop a simple, high-performance liquid chromatography (HPLC) method using a diode array detector for the quantification of predominant gingerols (4-, 6-, 8-, and 10-) and shogaols (6-, 8-, and 10-) in supercritical carbon dioxide extract of Halia bara. The optimum conditions for elution of seven compounds were: column, ODS Genesis; column temperature, 25 C; elution, gradient elution consisting of acetonitrile and aqueous formic acid; detection wavelength, 282 nm; flow rate, 1 mL/min; injection volume, 20 µL; and run-time, 38 min. The optimized HPLC method was validated for linear range, accuracy, precision (repeatability and reproducibility), sensibility (limit of detection and quantification) and recovery. All the validation parameters were within the permissible limits stipulated by the International Council of Harmonization guidelines. In Halia bara supercritical fluid-carbon dioxide extract, the amounts of bioactive constituents are in the decreasing order of 10-gingerol, 6-gingerol, 6-shogaol, 8- gingerol, 8-shogaol, 10-shogaol and 4-gingerol.