Abstract
A simple and reliable isocratic reversed phase high performance liquid chromatography (HPLC) method for the determination of enalaprilat in human plasma is described. After plasma extraction, enalaprilat is analyzed using a µ Bondapak C18 column kept at 50°C. The mobile phase consists of 60% A (0.01 M KH2PO4, pH 4.0) and 40% B (80% of 50:50 CH3CN:CH3OH + 20% A) v/v. Enalaprilat is monitored by UV detection at 205 nm. The method is quite simple and sufficiently sensitive, with a limit of quantitation of 5 ng/mL and limit of detection of 1 ng/mL; within day and between‐days assays showed a relative standard deviation (RSD) below 15%.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Liquid Chromatography & Related Technologies
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
