Abstract
Abstract A simple HPLC assay has been developed for free captopril in plasma and CSF, and for its mixed disulphides in CSF. Captopril is derivatized with p-bromophenacylbromide (p-BPB), and the adduct formed is extraced and then assayed using a reversed-phase column and UV detection. Captopril mixed disulphides are reduced with tributylphosphine to the free form which in turn is trapped with p-BPB. No clean-up procedures are necessary for this method which shows satisfactory accuracy and precision. A sensitivity level of 10 ng/mL has been reached for free total captopril in CSF, and for free captopril in plasma.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
