Abstract
High-performance liquid chromatography (HPLC) using a 205 nm UV detector was evaluated for usefulness in determining fatty acid compositions, especially unsaturated fatty acid compositions, of edible oils such as perilla, soybean and safflower oils, which mainly consist of triglycerides that contain mono and polyunsaturated fatty acids. Fatty acids were obtained by lipase-hydrolysis followed by petroleum ether extraction. Sample fatty acids were analyzed by HPLC without conversion to the derivatives. Butyric acid served as the internal standard for determination of fatty acids. The results showed close agreement with those of GC. The present method is applicable to the simple determination of fatty acid compositions, especially unsaturated fatty acid compositions, of edible oils without degradation due to heating and/or the oxidative deterioration of unsaturated fatty acids.
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