Abstract

Trehalose is an essential chemical marker to control a quality of the industrial yeast strains and to assess a tolerance of the yeasts products to different physical stresses. A high-performance liquid chromatography analysis with charged aerosol detection (HPLC–CAD) was developed for trehalose determination in industrial yeasts. The method offers a linearity in the range of 5.0–15mM with linear regression coefficient R2=0.9995, a good reproducibility and relatively short analysis time (7min). Trehalose can be detected at concentrations as low as 0.07mM, and limit of precise quantification is 0.2mM. The coefficient of variation (CV%) is 0.3%. The developed method is more sensitive compared with conventional chromatography procedure with UV absorbance detection. It was shown that the proposed method can be used in baker’s industry to control a quality of the yeast products and to assess biotechnological significance of the yeast strains.

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