Simple and sensitive method for the biological determination of histamine in small amounts of liquids

Abstract

A simple method for the determination of histamine—using inverted pieces of isolated guinea pig intestine—is described, whereby amounts as small as 0,2 ml of solution may be employed. The difference between this and other methods is that the solution is merely filled into the lumen of the inverted intestine instead of—as is generally done—bringing the solution into contact with it from the exterior. Since the response and sensitivity of the intestine towards histamine are not impaired by this technique, the determination of histamine in much smaller amounts of fluids than hitherto possible is now assured.