Abstract
A rapid and low-cost nano-drop spectrophotometric method using citrate-modified silver nanoparticles (Ag NPs) for the determination of thiol-containing toxicants was developed. The introduction of thioglycolic acid (TGA) and thiourea (TU) reduced the overall surface charge of Ag NPs, resulting in aggregation of Ag NPs, and a colorimetric response that was individually correlated with the concentration of TGA and TU. Under optimum experimental conditions, the maximum molar absorptivity values for TGA and TU were 1.04 × 105 and 2.13 × 105 L × mol–1 × cm–1, respectively, at λmax of 415 nm. The linear range used was 0.5–2.5 mg/L for TGA, and 0.3–1.5 mg/L for TU. The detection limits (3S) and % relative standard deviation (RSD) for the method were found to be 3 ppb, 2 ppb, and ±1.13%, ±0.96% for TGA and TU, respectively. This new chromogenic method provided a facile and sensitive scheme for the determination of TGA and TU, and could be applied for the determination of thiol-containing biomolecules. This scheme was tested for the analysis of real samples such as urine, blood, and environmental samples.
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