Abstract
The stromal vascular fraction (SVF) consists of a heterogeneous population of stem and stromal cells, generally obtained from adipose tissue by enzymatic digestion. For human cell-based therapies, mechanical process methods to obtain SVF represent an advantageous approach because they have fewer regulatory restrictions for their clinical use. The aim of this study was to characterize a novel commercial system for obtaining SVF from adipose tissue by a mechanical approach without substantial manipulations. Lipoaspirate samples collected from 27 informed patients were processed by a simple and fast mechanical system (by means of Hy-Tissue SVF). The Hy-Tissue SVF product contained a free cell fraction and micro-fragments of stromal connective tissue. The enzymatic digestion of the micro-fragments increased the yield of free cells (3.2 times) and CFU-F (2.4 times). Additionally, 10% of free cells from SVF were positive for CD34+, suggesting the presence of endothelial cells, pericytes, and potential adipose-derived stem cells (ADSC). Moreover, the SVF cells were able to proliferate and differentiate in vitro toward adipocytes, osteocytes, and chondrocytes. The immunophenotypic analysis of expanded cells showed positivity for typical mesenchymal stem cell markers. The Hy-Tissue SVF system allows the isolation of stromal vascular fraction, making this product of potential interest in regenerative medicine.
Highlights
IntroductionBiological therapies have become popular in the last few decades since they represent an alternative to the classical pharmacologic or surgical approaches to treat acute and degenerative chronic injuries
We have investigated the composition of the stromal vascular fraction (SVF) obtained with the Hy-Tissue SVF kit
22 lipoaspirates were processed following the protocol recommended by the manufacturer
Summary
Biological therapies have become popular in the last few decades since they represent an alternative to the classical pharmacologic or surgical approaches to treat acute and degenerative chronic injuries In this sense, adipose tissue has been postulated as an important biological product for the treatment of chronic and acute degenerative conditions since it is a source rich in adult multipotent stem cells [1]. Stromal Vascular Fraction (SVF) represents a freshly isolated heterogeneous cell mixture present in the adipose tissue. It comprises smooth muscle cells, preadipocytes, endothelial cells (ECs), and several multipotent adult stem cells, such as endothelial and hematopoietic progenitor cells (EPC), pericytes, and adipose-derived stem cells (ADSC), among others [2,3].
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