Simple and Rapid Methods for the Analysis of Captopril in Dosage Forms

H Chandru,A C Sharada

doi:10.1155/2007/915470

Abstract

Two indirect methods are described for the micro determination of captopril using hexacyanoferrate (III) as reagent. The reaction used for titrimetry proceeds at room temperature and will be complete in 10 minute with a stoichiometry of 1:1 with respect to the oxidant and captopril. The reaction product used for spectrophotometric determination shows the absorption maximum at 510 nm. The Beer’s law is obeyed over the concentration range 0.25-12.00 μg mL-1, the molar absorptivity and Sandell sensitivity for the system being 9.14×103L mol-1cm-1and 23.78 ng cm-2, respectively. The limit of detection and quantification are found to be 0.08 and 0.26 μg mL-1, respectively. Both procedures have been applied to the determination of captopril in tablets. The results have been statistically compared with those obtained by the official (BP) method.

Highlights

Captopril, 1-(3-mercapto-2-D-methyl-1-oxopropyl)-1-proline, is an antihypertensive drug widely prescribed in the treatment of hypertension and chronic congestive heart failure
A 3.0mL aliquot of standard drug solution containing 2.5-120 μg of captopril was placed in a 10 mL volumetric flask to which 1.5 mL of 5 M hydrochloric acid and 1mL of 100 μg mL-1 of hexacyanoferrate (III) solutions were added
Both methods are based on the oxidation of captopril with excess of hexacyanoferrate (III)

Summary

Introduction

1-(3-mercapto-2-D-methyl-1-oxopropyl)-1-proline, is an antihypertensive drug widely prescribed in the treatment of hypertension and chronic congestive heart failure. In search of more convenient methods, titrimetric and spectrophotometric investigations of the oxidation reaction between captopril and hexacyanoferrate (III) have been carried out and their application to the analysis of authentic samples and dosage forms have been examined.

Results

Conclusion