Simple and Noninvasive Breath Test Using 13C-Acetic Acid to Evaluate Gastric Emptying in Conscious Rats and Its Validation by Metoclopramide

Abstract

The 13C-breath test has been used to clinically evaluate gastric emptying. However, this method has not been sufficiently validated in experimental animals. The present study aimed to establish a simple and noninvasive 13C-breath-test system in Sprague-Dawley male rats. After fasting, rats were orally administered Racol containing 13C-acetic acid and housed in a desiccator. The expired air in the chamber was collected in a breath-sampling bag using a tube and aspiration pump. The level of 13CO2 in the expired air was measured using an infrared spectrometer at appropriate intervals for 120 min. During this period, the rate of 13CO2 excretion increased, peaked, and decreased thereafter. The maximum concentration (Cmax) and area under the curve (AUC120 min) of 13CO2 excretion increased in volume- and dose-dependent manners. The time taken to reach the maximum concentration (Tmax) of 13CO2 excretion increased as the volume increased, but was not affected by the dose of 13C-labeled acetic acid. Metoclopramide dose-dependently increased the Cmax and shortened Tmax of 13CO2 excretion compared with those of the control rats, whereas the AUC120 min was not affected. These results confirm that this simple method can successfully evaluate gastric emptying. Moreover, this system is suitable for investigating additional physiological functions using other labeled compounds.