Abstract
A thin enzyme-immobilized membrane was prepared from squid pen chitin under simple and mild conditions. Squid pen chitin had better physical properties than crab chitin owing to the β-type crystalline structure in the former. The membrane exhibited not only the ability to entrap enzyme molecules but also sufficient oxygen and glucose permeabilities useful for biosensors. A glucose sensor, which was constructed with glucose oxidase immobilized on the chitin membrane and an oxygen electrode, exhibited a wide range of linearity (0.125–2.0 mM) and possessed good stability.
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