Simple and Inexpensive Methods Development for Determination of Venlafaxine Hydrochloride from Its Solid Dosage Forms by Visible Spectrophotometry

L. Shanti Swarup,C. Ramdas,M. N. Rao,K. Raghubabu,B. Kalyanaramu

doi:10.1155/2012/908715

Abstract

Two simple, sensitive and cost effective visible spectrophotometric methods (M1 and M2) have been developed for the determination of venlafaxine hydrochloride from bulk and tablet dosage forms. The method M1 is based on the formation of green colored coordination complex by the drug with cobalt thiocyanate which is quantitatively extractable into nitro benzene with an absorption maximum of 626.4 nm. The method M2 involves internal salt formation of aconitic anhydride, dehydration product of citric acid [CIA] with acetic anhydride [Ac2O] to form colored chromogen with an absorption maximum of 561.2 nm. The calibration graph is linear over the concentration range of 10-50 µg/mL and 8-24 µg/mL for method M1 and M2 respectively. The proposed methods are applied to commercial available tablets and the results are statistically compared with those obtained by the reference method and validated by recovery studies. The results are found satisfactory and reproducible. These methods are applied successfully for the estimation of the venlafaxine hydrochloride in the presence of other ingredients that are usually present in dosage forms.

Highlights

Venlafaxine hydrochloride (VX) is a bicyclic third generation antidepressant chiral compound of phenethyl amine type with a novel chemical structure 1, 2 (Fig.[1]).1646 K
Among the various water immiscible organic solvents (C6H6, CHCl3, dichloro methane, nitro benzene, chloro benzene and CCl4) tested for the extraction of colored coordinate complex into organic layer, nitrobenzene was preferred for selective extraction of colored complex from organic phase in method M1
Different solvents like acetic anhydride, acetic acid, methanol, ethanol, and isopropanol were used as diluents but acetic anhydride was found to be ideal for final dilution in method M2

Summary

Introduction

Venlafaxine hydrochloride (VX) is a bicyclic third generation antidepressant chiral compound of phenethyl amine type with a novel chemical structure 1, 2 (Fig.[1]).1646 K. The drug is official in BP 3 and suggests potentiometric method for the determination of VX in bulk and tablet formulations.

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Results

Conclusion