Abstract
The objective was identifying three different anthocyanidins and to quantify them in six different berries. Results from the conventional method (using extraction with hydrochloric acid) were compared with data from the proposed method (without acid extraction). The following figures of merit were used: linearity, accuracy, linear range, limits of detection and quantification, using an ultra performance liquid chromatography (UPLC) coupled to a triple quadrupole mass spectrometer equipped with an electrospray ionization source. The separation of anthocyanidins can be achieved in a much smaller period of time (1.30 min) and the cyanidin from Morus nigra L., Rubus idaeus L. and Vaccinium myrtillus L. was found in higher amounts in extracts obtained from the proposed method. Proposed mechanisms of mass spectrometry (MS/MS) collisional induced dissociation for pelargonidin, cyanidin and delphinidin were obtained and this is the first time that concentrations of anthocyanidins present in fruits of Solanum americanum Mill. were reported.
Highlights
In order to perform the basic functions of nutrition, cell renewal and respiration, all plants perform photosynthesis, which involves the primary metabolism, characterized by essential biochemical transformations in the generation of energy, being indispensable the insertion of macronutrients in this metabolism.[1,2]Some plant species, in addition to primary metabolism, developed secondary metabolism, such development is dependent on the genetic predisposition of each plant organism
Phenolic compounds are derived from this secondary metabolism, mainly by the shikimic acid route and to a lesser extent by the mevalonic acid pathway, and they are subdivided into other important groups that vary according to the number of phenols present in the molecules
This result demonstrates that the separation of anthocyanidins can be achieved in a much smaller period of time (1.30 min) when compared to the conventional high pressure liquid chromatography (HPLC) runs, which are the most used for this purpose, with analytical runs of approximately 20 to 60 min.[11,13,14,15]
Summary
In order to perform the basic functions of nutrition, cell renewal and respiration, all plants perform photosynthesis, which involves the primary metabolism, characterized by essential biochemical transformations in the generation of energy, being indispensable the insertion of macronutrients in this metabolism.[1,2]Some plant species, in addition to primary metabolism, developed secondary metabolism, such development is dependent on the genetic predisposition of each plant organism. It is necessary to develop faster and more sensitive analytical methodologies for determining the concentrations of compounds with antioxidant properties, mainly in fruits, characterized as complex samples to be analyzed.[8]
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