Abstract
BackgroundIn recent days, solid-phase extraction methods are widely utilized for the extraction of drug molecules from plasma samples due to their easy operating procedures and low matrix effect. The trace-level solid-phase extraction of three structurally related antiepileptic drugs brivaracetam (BVC), eslicarbazepine acetate (ESL), and carbamazepine (CBZ) was investigated by using a magnetic porous material graphene oxide-β-cyclodextrin (MGO-CD). Morphology, magnetic properties, and structure of the synthesized MGO-CD were characterized by using FT-IR, SEM, XRD, and VSM.ResultsSolid-phase extraction (SPE) methods were used to extract the analytes from human plasma. Different extraction solvents such as acetonitrile (ACN), methanol (MeOH), acetone, chloroform (CHCl3), tertiary butyl diethyl ether (TBDE), and ethyl acetate (EtOAc) with variable polarities were used to extract drug molecules from MGO-CD. The linearity analysis showed good correlation coefficient values (R2) of 0.9989, 0.9995, and 0.9982 for BVC, ESL, and CBZ respectively. The LOD and LOQ ranges were found to be 6.14–28.32 ng mL−1 and 20.45–94.31 ng mL−1 respectively.ConclusionThe high accuracy and precision made the developed HPLC method with MGO-CD a suitable alternative for the bioequivalence study of BVC, ESL, and CBZ in human plasma. This developed HPLC-UV method has good efficiency for recoveries and good linearity and is simple to handle. And also, it gave low retention time for the three antiepileptic drugs within 8 min. It provides high efficiency for the extraction of trace-level substances from human plasma.
Highlights
In recent days, solid-phase extraction methods are widely utilized for the extraction of drug molecules from plasma samples due to their easy operating procedures and low matrix effect
Characterization of synthesized Magnetic graphene oxide (MGO)-CD The magnetic graphene oxide and cyclodextrin (MGO-CD) composite was synthesized as depicted in the experimental details
The material graphene oxide-β-cyclodextrin (MGO-CD) composite was characterized by FT-IR, powder X-ray diffraction (XRD), Scanning electron microscopy (SEM), and vibrational sample magnetometer (VSM)
Summary
Solid-phase extraction methods are widely utilized for the extraction of drug molecules from plasma samples due to their easy operating procedures and low matrix effect. Epilepsy is a chronic brain disease described by sudden and transient brain dysfunction caused by recurrent episodes of neurons in the brain [1,2,3]. It is identified with symptoms like seizures and should be treated immediately with a quick-acting antiepileptic drug (AED). CBZ is a mood-stabilizing and anticonvulsant tricyclic lipophilic drug This drug is the first one to treat epilepsy for psychomotor and partial onset of seizures and is used for variability of indications, including schizophrenia, attention-deficit hyperactivity disorder (ADHD), paroxysmal dangerous pain disorder, phantom limb disease, and post-traumatic stress syndrome [16,17,18]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
