Abstract
Asaccharolytic anaerobic gram-negative motile rods were isolated from periodontal pockets and root canals. Organisms were studied that were stimulated in broth by formate and fumarate but did not resemble described bacterial species. Most strains were straight rods that pitted agar plates. Cluster analysis of phenotypic features and DNA/DNA homologies separated the oral isolates into three groups of Wolinella, distinct from W succinogenes. Eight oral strains were identified as W recta. Close DNA/DNA homology between root canal and periodontal isolates suggested that the periodontal pocket is a likely source of strains in root canal infections. Asaccharolytic anaerobic gram-negative motile rods were isolated from periodontal pockets and root canals. Organisms were studied that were stimulated in broth by formate and fumarate but did not resemble described bacterial species. Most strains were straight rods that pitted agar plates. Cluster analysis of phenotypic features and DNA/DNA homologies separated the oral isolates into three groups of Wolinella, distinct from W succinogenes. Eight oral strains were identified as W recta. Close DNA/DNA homology between root canal and periodontal isolates suggested that the periodontal pocket is a likely source of strains in root canal infections.
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