Abstract
Isolates of atypical scrapie recently identified in sheep and goats in France were compared with Nor98 isolates reported in Norway. Western blot methods for characterization of the protease-resistant prion protein showed that all these isolates shared a unique biochemical signature: 5 groups of bands, including a characteristic band of apparent low molecular weight (11 kDa). This pattern could originate from the presence of 3 different protease cleavage products, including the 11 kDa most likely cleaved at both N- and C-sides of the protein. Genetic data, which strongly suggested the higher susceptibility of AHQ and AF141RQ animals in French cases, resembled earlier data from Nor98 scrapie.
Highlights
To cite this version: Jean Noël Arsac, Olivier Andréoletti, Jean-Marc Bilheude, Caroline Lacroux, Sylvie L
In this study we investigated a panel of 54 French atypical isolates from sheep (n = 51) and goats (n = 3) and compared their PrP genotypes and advanced PrPres biochemical signatures (WB electrophoretic mobility, epitope mapping, and PrPres deglycosylation pattern) with those of Nor98 cases
The prnp polymorphisms from these atypical cases were compared with a panel from animals with classic scrapie
Summary
To cite this version: Jean Noël Arsac, Olivier Andréoletti, Jean-Marc Bilheude, Caroline Lacroux, Sylvie L. Et al. Similar biochemical signatures and prion protein genotypes in atypical scrapie and Nor cases, France and Norway. Similar Biochemical Signatures and Prion Protein Genotypes in Atypical Scrapie and Nor Cases, France and Norway. Western blot methods for characterization of the protease-resistant prion protein showed that all these isolates shared a unique biochemical signature: 5 groups of bands, including a characteristic band of apparent low molecular weight (11 kDa). This pattern could originate from the presence of 3 different protease cleavage products, including the 11 kDa most likely cleaved at both N- and C-sides of the protein. Genetic data, which strongly suggested the higher susceptibility of AHQ and AF141RQ animals in French cases, resembled earlier data from Nor scrapie
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
