Abstract
The location of integrated SV40 DNA in a series of virus-free transformed murine cells was analyzed by characterizing the reiteration frequency of adjacent host cell DNA sequences. Cellular DNA was mechanically sheared to fragments approximately 8 × 10 6 or 7 × 10 5 daltons in size, separated into reiterated and nonreiterated fractions, and then examined for the presence of SV40 DNA. SV40 DNA sequences were associated with the nonreiterated fraction of mammalian DNA in each of the cell lines examined. In addition, the pattern of viral DNA distribution between the repetitive and unique cellular DNA fractions varied from cell line to cell line suggesting that integration of SV40 DNA may occur at different sites in each cell line examined.
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