Silvibacterium bohemicum gen. nov. sp. nov., an acidobacterium isolated from coniferous soil in the Bohemian Forest National Park

Abstract

During the course of a study assessing the bacterial diversity of a coniferous forest soil (pH 3.8) in the Bohemian Forest National Park (Czech Republic), we isolated strain S15T which corresponded to one of the most abundant soil OTUs. Strain S15T is represented by Gram-negative, motile, rod-like cells that are 0.3–0.5μm in diameter and 0.9–1.1μm in length. Its pH range for growth was 3–6, with optimal conditions found at approximately 4–5. It can grow at temperatures between 20°C and 28°C, with optimum growth at 22–24°C. Its respiratory quinone is MK-8, and its main fatty acid is iso-C15:0 (73.7%). The G+C DNA content was 58.2mol%. According to the 16S rRNA gene sequence analysis, strain S15T belongs to subdivision 1 of the phylum Acidobacteria, being affiliated to the cluster of Acidipila rosea AP8T and Acidobacterium capsulatum ATCC 51196T. Analysis of the S15T genome revealed the presence of 404 genes that are involved in carbohydrate metabolism, which indicates the metabolic potential to degrade polysaccharides of plant and fungal origin. Based on genotypic and phenotypic characteristics, the strain S15T represents a new genus and species within the family Acidobacteriaceae, for which the name Silvibacterium bohemicum gen. nov., sp. nov. is proposed (type strain S15T=LMG 28607T=CECT 8790T).