Abstract
AbstractThe voltammetric behavior of hydroxocobalamin (OH‐Cbl), one form of vitamin B12, has been studied using two modifications of silver solid amalgam electrode: polished (p‐AgSAE) and mercury meniscus modified (m‐AgSAE). All results were compared with these achieved using hanging mercury drop electrode (HMDE). Various voltammetric methods, like cyclic voltammetry, DC voltammetry, differential pulse voltammetry and elimination voltammetry with linear scan, were applied to investigate the electrochemical behavior of OH‐Cbl. Working conditions of differential pulse voltammetry were found and the achieved limits of detection (LD=1.5 nmol L−1 (m‐AgSAE) and 3.3 nmol L−1 (p‐AgSAE)) proved high sensitivity. The applicability of the proposed method was verified by analysis of vitamin preparation.
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