Abstract
Silver nanoparticles (AgNPs) are widely applied in many fields because of their excellent antibacterial activities. Toxicological studies have showed that AgNPs can cross the blood-brain barrier and exhibit high retention in the brain. Therefore, the potential neurotoxicity of AgNPs is raising serious concerns. This study investigated the neurotoxicological effects of AgNPs with two different sizes (20nm and 70nm, AgNPs-20 and AgNPs-70) using primary cultures of rat cerebral cortical neurons in mature and developing stages. The contribution of silver ion release was investigated by testing the effects of ionic silver in parallel. The results showed that both AgNPs-20 and AgNPs-70 significantly decreased neuronal cell viability, and AgNPs-20 had stronger toxicity compared with AgNPs-70. AgNP applications caused the granulated skeleton structure of the mature neurons with some broken synapses after a 24-h exposure, and inhibited neuronal growth during a 7-day exposure. Intracellular silver accumulation at non-cytotoxic exposure levels inhibited dopamine efflux, which was particle-specific and free of released silver ions. The findings herein can aid in guiding the proper applications of AgNPs in different areas, especially in medical use.
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