Abstract
Event Abstract Back to Event Silver nanoparticles in glass ionomer cements: surface characterization and validation of the antibacterial activity Lilian Paiva1, 2, Karine Anselme2, Lydie Ploux2 and Rossana Thiré1 1 Federal University of Rio de Janeiro, Metallurgical and Materials Engineering, Brazil 2 Institut de Science de Matériaux de Mulhouse, Biomaterials and interfaces, France Glass ionomer cements (GIC) are preventive materials commonly used in the treatment of dental caries. Reducing bacterial adhesion and consequently disrupting dental plaque over these materials shall be decisive trend, since their rough surfaces induce more cariogenic plaque formation in comparison with enamel and other dental materials[1]. On the other hand, GIC are considered an important bioactive material for caries management according to minimal invasive operative interventions. The aim of this study is to evaluate preventive bacterial adhesion properties of GIC containing silver nanoparticles (AgNP)[2]. Characterization of AgNP-GIC with increasing amount of silver salt (Without Ag, Low Ag, Medium Ag and High Ag) was pursued in order to verify any significant changes on their properties. Compressive strength (CS) and net setting reaction were tested according to ISO 9917:1. Biocompatibility was evaluated by indirect MTT assay, in which human gingival fibroblasts HGF-1 (ATCC® CRL-2014™) were cultivated using the 24 h DMEM extract of the AgNP-GIC. Streptococcus mutans biofilm formation (24 h) on the surfaces of AgNP-GIC were evaluated using in situ confocal laser scanning microscopy (CLSM) and images were further analyzed with Image J and COMSTAT2 software[3]. MTT assay measured viability of S. mutans on 24 h biofilms. Higher concentration of silver in the matrix increased compressive strength of the cements, due to the anchoring of polymer chains. Besides, although physical chemical properties such as net setting time and color were slightly affected by AgNP synthesis, GIC still remained within suitable parameters for clinical use. Although High Ag was efficient in disrupting S. mutans biofilm formation and viability, it also reduced HGF-1 viability due to silver content released. Medium Ag showed to be the concentration of silver salt in which bacterial adhesion and biofilm formation parameters (biomass, mean thickness, diffusion distance and surface to bio-volume) were significantly affected compared to control, causing formation of large aggregates or web-like biofilms, and cell viabilities remained unaltered. This study developed and characterized an experimental GIC containing AgNP, which cause disruption on biofilm formation[4]. Moreover, within the limitations of this study, it appears that the experimental cement is a clinically attractive dental material due to its ability of providing silver and fluoride ions to adjacent dental tissues[5], possibly also acting as a cariostatic agent. {{AbstractFigure.1}} {{AbstractFigure.2}} {{AbstractFigure.3}} Keywords: Bacteria, nanocomposite, in vitro, nanoparticle Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Biomaterials in dental applications Citation: Paiva L, Anselme K, Ploux L and Thiré R (2016). Silver nanoparticles in glass ionomer cements: surface characterization and validation of the antibacterial activity. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02921 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Lilian Paiva Karine Anselme Lydie Ploux Rossana Thiré Google Lilian Paiva Karine Anselme Lydie Ploux Rossana Thiré Google Scholar Lilian Paiva Karine Anselme Lydie Ploux Rossana Thiré PubMed Lilian Paiva Karine Anselme Lydie Ploux Rossana Thiré Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.